Dear all,

I have a question regarding a Split Plot ANOVA I had to calculate in STATISTICA:

In total, I analysed four factors: Two fixed factors: OAW and Nutrients, and two random factors: Mesocosms and Sibling group.

The factors OAW and N have two levels each and are fully crossed. Each OAWx N combination has three replicates, that are three mesocosms. So, for four treatment combinations I have 12 mesocosms in total.

I nested the factor mesocosm within the treatment combination OAWxN. This was what the reviewers asked me to do. Sibling group was just treated as a random factor.

So, the design in statistica was: oaw*N (mesocosm) + oaw + N + oaw*N + sib + n*sib + oaw*sib

The random factor mesocosm is statistically significant, so: OAW x N (mesocosm): p-value < 0.05

Does this mean, that the difference between mesocosm over all 12 mesocosms (and therefore over all 4 treatment combinations) are statistically significant? Or does this mean, that the difference of three mesocosms within each treatment combination is statistically different?

How should this be interpreted in the article? Is the OAW effect, which I found, still reliable?

Thanks in advance,

Bel